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Explain the various applications of fluorescence microscopy
Assessment Strategies
Oral presentation to class
Written exams
Criteria
oral presentation and written exams include the history of the evolution of fluoresence microscopy (FM)
oral presentation and written exams include the current status of FM
oral presentation and written exams include the future vision of FM
oral presentation and written exams include the discription of the fluorescence signal
oral presentation and written exams compare FM with Optical Light Microscopy (OLM)
oral presentation and written exams compare FM with Scanning Electron Microscopy (SEM)
oral presentation and written exams compare FM with Transmission Electron Microscopy (TEM)
oral presentation and written exams explain the limitations of FM
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Correlate type of specimen, desired information and applicable fluorophores.
Assessment Strategies
Skill demonstration in the lab
Written exams
Criteria
you identify specimen status
you determine the type of desired information from specimen
you select correct fluorophore
you achieve passing score on tests
your written product and exam includes identifying specimen status
your written product and exams include scope of information that can be analyzed from the specimen
your written product and exams include a description of the various fluorophores available
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Determine instrumentation parameters
Assessment Strategies
Skill demonstration in the lab
Written exams
Criteria
you determine the correct illumination source
you select the associated excitation filter
you select the associated barrier filter
you evaluate image data for best source
you compare various scan rates and image quality
you compare different detectors
you discuss the advantages and disadvantages of different detectors
your written product and exams include the correct illumination source
your written product and exams include selection of associated excitation filter
your written product and exams include selection of the associated barrier filter
your written product and exams include evaluation of image data for best source
your written product and exams include comparison of various scan rates and image quality
your written product and exams include a comparison of different detectors
your written product and exams include discussion of the advantages and disadvantages of different detectors
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Collect images
Assessment Strategies
Skill demonstration in the lab
Through a written product
Written exams
Criteria
you acquire image with acceptable signal to noise ratio
you develop efficient microscope operation skills
you differentiate between multiple fluorophore signals
you recognize collection artifacts
you identify cell structures which correspond to fluorophores used
you critique image characteristics
you determine set of acceptable digital filters
you apply digital filters with correct radius and strength
your written product and exams include how to acquire image with acceptable signal to noise ratio
your written product and exams include determining efficient microscope operation skills
your written product and exams include differentiating between multiple fluorophore signals
your written product and exams include recognizing collection artifacts
your written product and exams include identifying cell structures which correspond to fluorophores used
your written product and exams include critiquing image characteristics
your written product and exams include determining set of acceptable digital filters
your written product and exams include applying digital filters with correct radius and strength
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Perform lab procedures to biological specimens for SEM examination
Assessment Strategies
Skill demonstration in the lab
Through a written product
Written exams
Criteria
you prepare specimen using chemical processing
you prepare specimens using Critical Point Drying (CPD) methods
you prepare specimens using Freeze Drying (FD) methods
you mount specimens for Scanning Electron Microscope (SEM) observation
you coat specimens using plasma magnetron techinques
you assess specimen preparation efficacy
your written product and exams include a description of chemical processing
your written product and exams include a description of Critical Point Drying (CPD) methods
your written product and exams include a description of Freeze Drying (FD) methods
your written product and exams include a description of mounting specimens for Scanning Electron Microscope (SEM) observation
your written product and exams include a description of coating specimens using plasma magnetron techinques
your written product and exams include assessment of specimen preparation efficacy
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Evaluate specimen preparation efficacy
Assessment Strategies
Skill demonstration in the lab
Through a written product
Written exams
Criteria
you select SEM microscope parameters for sample conditions
you operate SEM to obtain desired specimen data
you evaluate specimens' fine structure integrity
your written product and exams include parameters for the SEM microscope regarding sample conditions
your written product and exams include SEM operaton procedures in order to obtain desired specimen data
your written product and exams include evaluation of specimens' fine structure integrity
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Produce cell cultures
Assessment Strategies
Skill demonstration in the lab
Through a written product
Written exams
Criteria
you apply aseptic techniques to obtain contamination-free cell cultures
you isolate cells
you use fermentation technology to cell cultures
you grow industrial microorganisms
you preserve industrial microorganisms
your written product and exams include descriptions of aseptic techniques to obtain contamination-free cell cultures
your written product and exams include procedures to isolate cells
your written product and exams include description of fermentation technology to cell cultures
your written product and exams include conditions needed to grow industrial microorganisms
your written product and exams include conditions needed to preserve industrial microorganisms
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Examine cell cultures
Assessment Strategies
Skill demonstration in the lab
Through a written product
Written exams
Criteria
your media is sterile
your cultured cells remain viable
you clone cell cultures
you produce monoclonal antibody production
your written product and exams include sterile media preparation techniques
your written product and exams include how to maintain viable cultured cells
your written product and exams include how to clone cell cultures
your written product and exams include how to produce monoclonal antibody production