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Analyze the classification of plants and their structure
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Outline the characteristics of vascular and nonvascular plants
Examine the basic plant life cycle
Explore mitosis and meiosis
Compare and contrast dicots and monocots
Identify parts of the whole plant structure
Identify reproductive parts of plant
Explain the basic structure and function of leaves, stems, and roots
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Maintain plant cultures with no contamination
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Explain the function of each media component
Explore how plant hormones affect plant growth and organogenesis
Describe how to prepare media aseptically
Perform a bioassay for cytokinin activity
Calculate and plot plant cell growth by determining root and shoot growth
Dissect and culture parts and embryonic tissue of plants using bright-field microscopy and open-bench aseptic technique
Outline, establish and maintain callus, meristem, and plant cell suspension culture
Explain the basic causes of explant loss
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Examine genetic transformation of plants
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Explain the methods used for recombinant DNA transfer with plans including the use of Agrobacterium, electroporation and microprojectile bombardment
Examine a basic technique for isolating DNA from plant material
Demonstrate Agrobacterium-mediated transformation of plant tissue cultures
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Analyze the historical, present, and future aspects of animal cell culture
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Identify the major historic advances in cell culture
Explain the advantages and disadvantages of cell culture
Summarize the different types of cultures
Examine the anatomy and function of subcellular organelles
Examine the elements of selection in the origin and evolution of cell lines
Explain the cell cycle and the signals from the environment that regulate it
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Implement laboratory safety and biohazard procedures
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Identify typical general safety hazards in a tissue culture laboratory
Demonstrate an effective level of protection for various biohazard procedures
Demonstrate effective disposal procedures for tissue culture materials
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Determine the equipment needs of an animal cell culture lab
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Confirm essential and beneficial equipment for a cell culture facility
Identify other types of equipment that may be found in a cell culture facility
Determine the necessary consumable items used in a tissue cell facility
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Demonstrate aseptic technique
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Describe the objectives of aseptic technique using biological safety cabinets (BSC)
Demonstrate good technique for work surface, personal hygiene, pipetting, and sterile handling
Explain the mechanism of biological safety cabinets (BSC)
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Prepare media for culturing cells
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Compare the surfaces and the dishes, plates, and vessels that cells will grow on
Explain the relationship between CO2, temperature, buffering, and pH
Explain the basic constituents of media
Contrast the advantages and disadvantages of serum-free media
Clean and sterilize equipment
Prepare media in biological safety cabinets (BSC)
Prepare media and sterilize by filtration
Test media for sterility
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Maintain and passage cultured mammalian cells
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Culture CHO-KI, iPS (IMR90)-4, and 40-1a cell lines without contamination in biological safety cabinets (BSC)
Culture cells in various kinds of incubation vessels
Count cells using a hemocytometer
Count cells using an automated cell counter (Countess)
Calculate and prepare dilutions of cells
Plate cells at a specific cell density
Demonstrate cryopreservation techniques by freezing and thawing CHO-KI and iPS (IMR90)-4 cells
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Apply methods for manipulating and analyzing cultured cells
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Explain morphology and health of cell cultures using bright-field microscopy
Describe methods for DNA, RNA and protein analysis of harvested cells
Demonstrate how cell cultures can be used to assay viability, growth, and cytotoxicity
Transfect CHO-KI cells with plasmid DNA
Select and clone transfected cells
Document the expression of recombinant protein in transfected cells using fluorescent microscopy
Examine the basic biology of B cells and T cells
Diagram an antibody molecule
Compare polyclonal and monoclonal antibodies
Examine the methods and uses for antibodies in ELISA, dot-blot, Western Blot, and hybridoma production
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Explain industrial aspects of cell culturing
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Describe industrial applications such as biopharmaceutical production, vaccine development, drug discovery, and food and beverage production
Identify various products from cultured animal cells, such as the production of monoclonal antibodies, viral vaccines, or fermented foods
Identify the basic components of a bioreactor
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Analyze the production and use of induced pluripotent stem cells in medical and research applications
Assessment Strategies
Written Product, Exam, and/or Skill Demonstration in Lab
Criteria
Compare the morphology of somatic and stem cells using bright-field microscopy
Investigate current methods for the maintenance and use of induced pluripotent stem cells (IMR90-4)
Explain differentiation methods and states for stem cell cultures