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Demonstrate aseptic technique and sterility control procedures
Assessment Strategies
Laboratory activity: Completion of written observations
Criteria
Select the desired microorganisms
Consistently sterilize all materials used in the fermentation process
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Evaluate various bioprocessing applications
Assessment Strategies
Laboratory performance, Written assignment
Criteria
Define “bioprocessing” and “fermentation”
Identify cell types and microorganisms relevant to bioprocessing
Explain the steps and equipment in industrial bioprocessing
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Identify local companies that are part of the bioprocessing industry
Assessment Strategies
Oral presentation, Written assessment
Criteria
Identify at least three local companies that are involved in bioprocessing
Describe the types of jobs found at these companies and the skill sets required to be successful in these jobs
Summarize the products produced by these companies and their relevance to customers
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Describe sources for identification of industrially-relevant microbes
Assessment Strategies
Laboratory activity/performance, Written assessment
Criteria
Describe various sources commonly mined for microbes with industrially relevant functions
Describes at least three relevant industrial products that have been identified through bioprospecting
Describe methods for primary, and secondary screening, and strain improvement
Perform a primary screen and analyze relevant laboratory data
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Identify parameters to be optimized during process development
Assessment Strategies
Laboratory experiment: written observations and results
Written assessment
Presentation of laboratory data
Criteria
Describe the various steps and functions involved in upstream and downstream bioprocessing
Experimentally optimize parameters in a bioprocessing process
Analyze and explain data to determine optimal growth conditions
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Describe methods to determine cell growth
Assessment Strategies
Laboratory experiment/performance
Written assessment
Written documentation of results analysis
Criteria
Identify the different methods to determine cell density in a cell culture
Differentiate between direct and indirect methods of cell counting
Create a standard curve relating cell density spectrophotometrically to cell concentration
Determine cell concentration of several cultures
Describe the stages in the growth curve of a culture
Calculate the generation time and growth rate of several cultures
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Prepare, assemble and effectively use fermentation equipment
Assessment Strategies
Written product
Laboratory exercises/performance
Written documentation of results analysis
Criteria
Identify the parts of a fermentation vessel and describe the functions of those parts
Set up and take down a fermentation apparatus
Use several types of fermentation vessels to grow cells
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Implement scale-up of a fermentation process
Assessment Strategies
Laboratory exercises/performance
Written product
Documentation of results analysis
Criteria
Define “scale-up”
Discuss potential difficulties with the scaleup of a bioprocessing process
Sale up a small-scale fermentation process
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Perform process optimization
Assessment Strategies
Written product
Laboratory exercises/performance
Written documentation of results analysis
Oral summary/recommendation report
Criteria
Identify parameters to optimize in the laboratory
Design an upstream bioprocessing scheme
Perform a process optimization scheme
Quantify the product obtained in the fermentation process
Report optimal conditions and/or next steps
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Demonstrate a biosafety mindset
Assessment Strategies
Laboratory activities/performance
Written product
Criteria
Describe procedures to minimize risks associated with biohazards in the biotechnology laboratory
Identify and use appropriate personal protective equipment
Identify the risks associated with working with various biohazards encountered in a biomanufacturing facility
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Design various fermentation media for specific fermentation applications
Assessment Strategies
Laboratory exercise/performance
Written product
Criteria
Identify media components that affect production and biomass formation
Compare media formulations to yield data
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Demonstrate documentation practices aligned with industry standards
Assessment Strategies
Laboratory notebook assessment
Written products
Criteria
Follow good documentation practices in all laboratory settings
Describe the principles of data integrity in the life sciences industry