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Perform a self-directed PCR-based cloning exercise
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies success cloning of PCR sequence of interest
Laboratory notebook clearly outlines cloning strategy
Laboratory notebook analyzes all data collected in lab and uses data to assess progress throughout plan
Laboratory notebook documentation follows industry-accepted standards
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Assess pipetting accuracy and precision using quantitative PCR (qPCR)
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies successful performance of a simple, preliminary qPCR exercise
Skills demonstration practices proper set-up of a dilution scheme
Skills demonstration displays accuracy and precision in micropipetting
Laboratory notebook documentation analyzes all data collected in lab and uses data to assess accuracy and precision of micropipetting
Laboratory notebook documentation follows industry-accepted standards
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Quantify the level of genetic modification in foods using quantitative PCR (qPCR)
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies successful isolation of DNA from food samples
Skills demonstration practices proper set-up of qPCR samples and controls
Laboratory notebook documentation analyzes all data collected in lab and uses data to evaluate the level of genetic modification in food samples relative to standards
Laboratory notebook documentation follows industry-accepted standards
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Analyze gene expression using reverse transcriptase-PCR (RT-PCR)
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies successful isolation of RNA
Skills demonstration practices evaluation of concentration, purity, and integrity of RNA product
Skills demonstration exhibits successful amplification of PCR product beginning with an RNA template
Laboratory notebook documentation analyzes all data collected in lab and uses data to evaluate the success of RT-PCR trials
Laboratory notebook documentation follows industry-accepted standards
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Design effective primers to analyze gene expression
Assessment Strategies
Laboratory skills demonstration
Written report
Criteria
Skills demonstration verifies use of bioinformatics tools to design primers
Skills demonstration tests success of primer design in the analysis of gene expression of target genes
Skills demonstration exhibits successful amplification of PCR product beginning with an RNA template
Written report clearly explains the reasoning for specific primer design choices
Written report analyzes RT-PCR data to describe relative levels of target gene expression
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Perform site-directed mutagenesis to restore function of a gene
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies use of site-directed mutagenesis to restore gene function
Laboratory notebook documentation analyzes all data collected in lab and uses data to evaluate the success of site-directed mutagenesis
Laboratory notebook documentation follows industry-accepted standards
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Use the CRISPR-Cas9 system to restore function of a gene
Assessment Strategies
Laboratory skills demonstration
Laboratory notebook documentation
Criteria
Skills demonstration verifies use of CRISPR-Cas9 to restore function of a gene
Laboratory notebook analyzes all data collected in lab and uses data to evaluate the success of CRISPR-Cas9 mediated mutagenesis
Laboratory notebook documentation follows industry-accepted standards
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Analyze contemporary and historical systems that structure and reproduce inequalities within the field of biotechnology
Assessment Strategies
Individual reflections
Group oral presentation
Criteria
Reflections exhibit understanding of historical cases of inequity with respect to, and exploitation of, non-dominant groups related to medical advances and human testing
Reflections relate historical inequities in the biotechnology field to inequities in current technologies and access to therapies
Group oral presentation identifies future problems with equitable access to cutting edge technologies in the biotechnology field
Group oral presentation discusses possible solutions to improve equitable access to cutting edge technologies and therapies