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1.1 Describe the historical developments that led to modern recombinant DNA technology
Assessment Strategies
Written Product, Exam
Criteria
describe the work of scientists discussed in their textbook and in computer animations
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2.1 Apply basic concepts of recombinant DNA technology
Assessment Strategies
Formal Lab Report, Exam
Criteria
diagram the relationship between DNA, RNA and protein
explain the basic Boyer-Cohen cloning experiment
define all basic components of cloning including vectors, restriction enzymes, host transformation and electrophoresis
apply these concepts to a simulated application
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2.2 Perform a DNA cloning exercise
Assessment Strategies
Lab Activities, Written Product
Criteria
clone a gene for antibiotic resistance into a vector so that transformed E. coli acquire antibiotic resistance
prepare written documentation (report) on cloning exercise
correctly answer questions in DNA Science text
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2.3 Perform a DNA mapping exercise
Assessment Strategies
Lab Activities, Written Product
Individual Proficiency Project
Criteria
transform plasmid DNA into an E.coli strain
perform plasmid purification from a transformed E. coli strain
perform restriction digest and electrophoresis
prepare a graph
use results to correctly determine the molecular weights of fragments
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3.1 Perform exercises involving PCR to study forensic analysis
Assessment Strategies
Lab Activities, Written Product
Criteria
DNA is successfully isolated from cheek cell swabs
gel is prepared with correct pattern
explain gel correctly in a written report
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3.2 Perform an exercise involving PCR to study genetic modification of foods
Assessment Strategies
Lab Activities, Written Product
Criteria
DNA is successfully isolated from food products
gel is prepared with correct pattern
explain gel correctly in a written report
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4.1 Apply PCR to a cloning exercise
Assessment Strategies
Lab Activities, Written Report
Criteria
DNA is successfully amplified from a bacteriophage genome
amplified DNA is successful inserted into a plasmid vector
recombinant plasmid is successfully transformed into E. coli
colony screening and PCR amplification is used to verify successful insertion
explain results in a written report
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4.2 Apply the concepts of screening, genetic expression, expression vectors, and genetic libraries
Assessment Strategies
Group Oral Presentation
Criteria
explain concepts in the context of a commercial molecular biology kit