Course | Madison College Outline of Instruction

10007105Bioprocess Technology

Course Information

Description

Covers basic techniques of fermentation technology, including the principles of isolation, identification, improvement, preservation and growth of industrial microorganisms. Emphasizes the use of fermentation equipment to obtain products.

Total Credits

Course Competencies

Evaluate various fermentation and bioprocessing applications

Assessment Strategies

by written analyses you prepare

Criteria

you explain the various product types to the class of fermentation or bioprocess

you identify various product types as to the class of fermentation or bioprocess
Explain the effects of specialized fermentation operations on the cell growth kinetics

Assessment Strategies

by diagramming the fermentation process relative the cell growth kinetics

Criteria

you explain the advantages of each fermentation process

you explain the disadvantages of each fermentation process
Validate the principles and importance of sterility in industrial fermentations

Assessment Strategies

by written explanation of sterility

by sterilizing equipment

by completing appropriate calculations and applying statistical methods to collected data

Criteria

you explain the important features of aseptic technique in terms of absolute sterility
Demonstrate aseptic technique and sterility control procedures

Assessment Strategies

when only desired microorganisms grow

Criteria

you aseptically transfer microorganisms

you sterilize all materials to be used in the fermentation process
Measure microbial growth

Assessment Strategies

by completion of laboratory activities

by completion of written explanation of laboratory observations and data

by completion of written assignments

Criteria

you remove samples and record growth parameters

you record growth parameters

you explain the results obtained in a growth experiment
Analyze fermentation samples

Assessment Strategies

through completion of laboratory activities

through written analysis of laboratory observations and data

through the completion of written assignments

Criteria

you assemble needed supplies and materials

you complete analysis of fermentation samples
Measure substrate and/or product using standard methods

Assessment Strategies

through the completion of laboratory activities

through the written analysis of laboratory observations and data

through the completion of written assignments

through written examinations

Criteria

you select the standard methods to be used

you assemble the needed materials and supplies

you measure the substrate and/or product in question

you explain observations and data
Summarize the theory of operation of a bench-top fermenter

Assessment Strategies

through the completion of written assignments

through written examinations

through oral examinations

Criteria

you summarize the function of parts of a bench-top fermenter

you summarize the function of the parts of a bench-top fermenter
Prepare bench-top fermenter for use

Assessment Strategies

through the completion of laboratory activities

through written explanations of laboratory observations and data

through the completion of written assignments

through written examinations

through laboratory proficiency examinations

Criteria

you assemble needed materials and supplies

you sterilize media and equipment as needed

you employ safety precautions as needed
Recover spent fermentation broth

Assessment Strategies

through the completion of laboratory activities

through the written explanations of laboratory observations and data

through the completion of written assignments

through written examinations

Criteria

you assemble needed supplies and materials

you initiate the appropriate process for the fermentation system used

you recover the spent broth for reuse or analysis
Calculate the efficiency of a fermentation process

Assessment Strategies

through the written evaluations of laboratory observations and data

through the completion of written assignments

through written examinations

Criteria

you identify the factors determining the efficiency of the process

you apply the appropriate mathematical functions to the calculations
Explain the effects of oxygen tension on product and biomass formation

Assessment Strategies

through the completion of written assignments

through written examinations

through written analyses of laboratory observations and data

Criteria

learner explains the effects of oxygen tension on product and bomass formation
Construct and test a galvanic dissolved oxygen probe

Assessment Strategies

through the completion of laboratory activities

through written explanation of laboratory observations and data

through written examinations

Criteria

your galvanic DO probe is completed

your galvanic DO probe measures dissolved oxygen
Calibrate polarographic and galvanic dissolved oxygen probes

Assessment Strategies

through the completion of laboratory activities

through written explanations of laboratory observations and data

through written examinations

Criteria

you calibrate the oxygen probes using standard industry protocols

your oxygen probes are within industry tolerance standards
Describe the relationship of oxygen transfer rates to mass transfer

Assessment Strategies

through the completion of written assignments

through written examinations

through the analysis of laboratory data

Criteria

you summarize the factors promoting oxygen transfer

you summarize factors inhibiting oxygen transfer

you differentiate oxygen transfer rates and mass transfer
Recover a microorganism from a lyophilized preparation

Assessment Strategies

through the completion of laboratory activities

through written analyses of laboratory observations and data

growth of microorganism with no contamination (pure culture)

Criteria

you rehydrate cell line

your microorganism grows in appropriate media
Determine the viability of stored cells

Assessment Strategies

through the completion of laboratory activities

through the written analyses of the laboratory observations and data

Criteria

you assemble the appropriate materials and supplies

you calculate the viability rates of stored cells

you grow cells on appropriate media
Explain the methods used to acquire and develop microbial strains for industrial uses

Assessment Strategies

through the completion of written assignments

through written examinations

through the assessment of journal articles identifying novel strains for industrial use

Criteria

you identify the methods used to develop microbial strains for industrial use

you explain the properties of microorganisms useful to industry
Design various fermentation media for specific applications for determining media factors affecting production/biomass formation

Assessment Strategies

through the completion of written assignments

through the written analysis of laboratory data

through written examinations

Criteria

you identify media components that affect production and biomass formation

you compare media formulations to yield data
Prepare analytical standards and establish an analytical calibration curve

Assessment Strategies

through the completion of laboratory activities

through written analysis of laboratory observations and data

through the completion of written assignments

through written examinations

through laboratory proficiency examinations

Criteria

your standard curve is prepared

you assemble the needed materials and supplies

you identify the appropriate analytical test to complete
Aseptically withdraw and prepare a sample for analysis

Assessment Strategies

through the completion of laboratory activities

through laboratory proficiency examinations

through written analysis of laboratory observations and data

Criteria

you apply standard aseptic techniques

your sample is not contaminated
Operate a fermentation high performance liquid chromatography system

Assessment Strategies

through the completion of laboratory activities

through laboratory proficiency examinations

through written explanations of laboratory observations and data

Criteria

you assemble the needed supplies and materials

you obtain a separation of molecules in sample

HPLC operates within the parameters identified
Comply with regulatory and ecological guidelines in the operation and design of fermentation processes

Assessment Strategies

through the completion of laboratory activities

through written examination

through laboratory proficiency examinations

Criteria

you follow applicable guidelines