10007104Chromatography Techniques
Course Information
Description
Introduces the basic concepts involved in separation of biomolecules. Students complete lab work using a variety of chromatographic methods including: paper, thin layer, gel permeation, gas and high performance liquid chromatography. Students also learn to interpret chromatographic results and practice documentation and reporting skills.
Total Credits
3
Course Competencies
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Apply basic chromatographic theoryAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner examines the relationship between chromatography and biphasic separations.learner classifies chromatographic methods by type as explained in text and lecture.learner selects the appropriate chromatographic method for given circumstances.learner shows diagrammatically a stationary and mobile phase, column and fractions.
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Interpret chromatographic resultsAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner performs qualitative and quantitative analysis to determine the amount and type of an unknown sample when given a chromatogram that includes proper standards and informationlearner detects uncertainties in the analysis and points out ways to confirm the analysis
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Judge the quality of a chromatographic separationAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner calculates efficiency and selectivity, identify tailing and frontinglearner uses these measures to analyze the ability of the method to separate a compound of interest when given a chromatogram
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Explain the importance of values in bioseparation techniquesAssessment Strategiesthrough the completion of written assignmentsthrough the completion of laboratory activitiesthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner explains the goals of bioseparation techniques relative to analysis and productionlearner explains the importance of quality control and professional ethics in disciplines that use bioseparation techniques
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Conducts lab preparation and record keepingAssessment Strategiesthrough the completion of written assignmentsthrough the completion of laboratory activitiesthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner prepares run sheets in lab notebook before all labslearner maintains proper records in notebook during labslearner prepares formal lab reports on time
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Apply the basic concepts of biphasic separationAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsCriterialearner predicts the separation properties of a compound in a polar/nonpolar system based on the compound's propertieslearner explains the technique of organic extractionlearner predicts separation properties quantitatively by applying a distribution coefficient equation
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Apply basic sampling principlesAssessment Strategiesthrough the completion of written assignmentsthrough the completion of laboratory activitiesthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner documents sample collectionlearner assigns identification information to a samplelearner samples a non-homogeneous mixture by collecting multiple sub-sampleslearner explains factors that govern sample collection
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Perform paper and thin layer chromatographyAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner separates test compounds in lab and correctly identifies unknowns using thin layer chromatographylearner separates test compounds in lab and correctly identifies unknowns using two dimensional paper chromatographylearner keeps lab notebook with summary of procedure and all results
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Interpret results of paper and thin layer chromatographyAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsCriterialearner explains the advantages of two dimensional chromatographylearner analyzes Rf values on chromatograms correctly
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Run a silica gel columnAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner pours silica gel columnlearner packs silica gel columnlearner runs silica gel columnlearner successfully separates mixture of organic compoundslearner collects fractionslearner evaluates fractionslearner keeps lab notebook with summary of procedure and all results
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Run a gel permeation separationAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner pours a gel permeation chromatography columnlearner packs a gel permeation chromatography columnlearner runs a gel permeation chromatography columnlearner successfully separates a test mixture into its componentslearner collects fractionslearner evaluates the fractions using a spectrophotometer
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Interpret results of gel permeation separationAssessment Strategiesthrough the completion of written assignmentsthrough the completion of laboratory activitiesthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner explains results in terms of molecular weight of sample componentslearner identifies unknown correctlylearner evaluates chromatograms using measures in competencies 1.2 and 1.3
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Report results of gel permeation separationAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner documents results of gel permeation separation in a lab notebook with summary of procedure and all resultslearner prepares formal report that summarizes interpretation of results in tabular and text form
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Perform proper maintenance and operation techniques to HPLC systemAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner attaches proper column to systemlearner checks for leakslearner checks pressurelearner primes pumpslearner runs test samplelearner stores column in correct storage solutionlearner identifies components of HPLC system and traces flow of liquid
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Run a sample on HPLC systemAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner obtains correct separation of sample components
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Interpret results of reverse phase column and sample interactionsAssessment Strategiesthrough the completion of written assignmentsthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner explains results in terms of reverse phase column and sample interactionslearner identifies unknown correctlylearner evaluates chromatograms using measures in competencies 1.2 and 1.3
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Document and report results of reverse phase column and sample interactionsAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner documents results of reverse phase column and sample interactions in a lab notebook with summary of procedure and all resultslearner prepares formal report that includes summarizes interpretation of results in tabular and text form
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Perform gas chromatography using standard and capillary columns and two types of detectorsAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observation of laboratory activitiesCriterialearner separates test compounds in lab using both Gow Mac and Hewlett Packard gas chromatographslearner correctly identifies unknowns
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Interpret results of gas chromatographyAssessment Strategiesthrough the completion of written assignmentsthrough the completion of laboratory activitiesthrough written examinationsthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner explains results in terms of sample and column polarity and interactionslearner correctly evaluates chromatograms using measures in competencies 1.2 and 1.3
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Document and report results of gas chromatographyAssessment Strategiesthrough the completion of laboratory activitiesthrough the completion of written explanations of results and observations of laboratory activitiesCriterialearner documents results of of gas chromatography in a lab notebook with summary of procedure and all resultslearner prepares formal report that includes summarizes interpretation of results in tabular and text form