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Apply basic chromatographic theory
Assessment Strategies
through the completion of written assignments
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner examines the relationship between chromatography and biphasic separations.
learner classifies chromatographic methods by type as explained in text and lecture.
learner selects the appropriate chromatographic method for given circumstances.
learner shows diagrammatically a stationary and mobile phase, column and fractions.
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Interpret chromatographic results
Assessment Strategies
through the completion of written assignments
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner performs qualitative and quantitative analysis to determine the amount and type of an unknown sample when given a chromatogram that includes proper standards and information
learner detects uncertainties in the analysis and points out ways to confirm the analysis
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Judge the quality of a chromatographic separation
Assessment Strategies
through the completion of written assignments
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner calculates efficiency and selectivity, identify tailing and fronting
learner uses these measures to analyze the ability of the method to separate a compound of interest when given a chromatogram
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Explain the importance of values in bioseparation techniques
Assessment Strategies
through the completion of written assignments
through the completion of laboratory activities
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner explains the goals of bioseparation techniques relative to analysis and production
learner explains the importance of quality control and professional ethics in disciplines that use bioseparation techniques
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Conducts lab preparation and record keeping
Assessment Strategies
through the completion of written assignments
through the completion of laboratory activities
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner prepares run sheets in lab notebook before all labs
learner maintains proper records in notebook during labs
learner prepares formal lab reports on time
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Apply the basic concepts of biphasic separation
Assessment Strategies
through the completion of written assignments
through written examinations
Criteria
learner predicts the separation properties of a compound in a polar/nonpolar system based on the compound's properties
learner explains the technique of organic extraction
learner predicts separation properties quantitatively by applying a distribution coefficient equation
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Apply basic sampling principles
Assessment Strategies
through the completion of written assignments
through the completion of laboratory activities
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner documents sample collection
learner assigns identification information to a sample
learner samples a non-homogeneous mixture by collecting multiple sub-samples
learner explains factors that govern sample collection
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Perform paper and thin layer chromatography
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner separates test compounds in lab and correctly identifies unknowns using thin layer chromatography
learner separates test compounds in lab and correctly identifies unknowns using two dimensional paper chromatography
learner keeps lab notebook with summary of procedure and all results
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Interpret results of paper and thin layer chromatography
Assessment Strategies
through the completion of written assignments
through written examinations
Criteria
learner explains the advantages of two dimensional chromatography
learner analyzes Rf values on chromatograms correctly
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Run a silica gel column
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner pours silica gel column
learner packs silica gel column
learner runs silica gel column
learner successfully separates mixture of organic compounds
learner collects fractions
learner evaluates fractions
learner keeps lab notebook with summary of procedure and all results
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Run a gel permeation separation
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner pours a gel permeation chromatography column
learner packs a gel permeation chromatography column
learner runs a gel permeation chromatography column
learner successfully separates a test mixture into its components
learner collects fractions
learner evaluates the fractions using a spectrophotometer
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Interpret results of gel permeation separation
Assessment Strategies
through the completion of written assignments
through the completion of laboratory activities
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner explains results in terms of molecular weight of sample components
learner identifies unknown correctly
learner evaluates chromatograms using measures in competencies 1.2 and 1.3
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Report results of gel permeation separation
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner documents results of gel permeation separation in a lab notebook with summary of procedure and all results
learner prepares formal report that summarizes interpretation of results in tabular and text form
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Perform proper maintenance and operation techniques to HPLC system
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner attaches proper column to system
learner checks for leaks
learner checks pressure
learner primes pumps
learner runs test sample
learner stores column in correct storage solution
learner identifies components of HPLC system and traces flow of liquid
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Run a sample on HPLC system
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner obtains correct separation of sample components
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Interpret results of reverse phase column and sample interactions
Assessment Strategies
through the completion of written assignments
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner explains results in terms of reverse phase column and sample interactions
learner identifies unknown correctly
learner evaluates chromatograms using measures in competencies 1.2 and 1.3
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Document and report results of reverse phase column and sample interactions
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner documents results of reverse phase column and sample interactions in a lab notebook with summary of procedure and all results
learner prepares formal report that includes summarizes interpretation of results in tabular and text form
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Perform gas chromatography using standard and capillary columns and two types of detectors
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observation of laboratory activities
Criteria
learner separates test compounds in lab using both Gow Mac and Hewlett Packard gas chromatographs
learner correctly identifies unknowns
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Interpret results of gas chromatography
Assessment Strategies
through the completion of written assignments
through the completion of laboratory activities
through written examinations
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner explains results in terms of sample and column polarity and interactions
learner correctly evaluates chromatograms using measures in competencies 1.2 and 1.3
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Document and report results of gas chromatography
Assessment Strategies
through the completion of laboratory activities
through the completion of written explanations of results and observations of laboratory activities
Criteria
learner documents results of of gas chromatography in a lab notebook with summary of procedure and all results
learner prepares formal report that includes summarizes interpretation of results in tabular and text form